Presented by

                     THE ROBERT CATHEY RESEARCH SOURCE

                        http://www.europa.com/~rsc

---------------------------------------------------------------------------
From: The Death of Cancer,
by Dr. Harold W. Manner, Ph.D.,
Steven J. DiSanti, and Thomas L. Michalsen
Advanced Century Publishing Corp., Chicago, USA
1979
pp. 177-179
---------------------------------------------------------------------------
TRANSMITTAL FORM FOR PAPERS

ILLINOIS STATE ACADEMY OF SCIENCE
ILLINOIS STATE UNIVERSITY

April 21, 22, 1978
Normal, Illinois

NON-TOXICITY OF AMYGDALIN TO MOUSE FETUSES,

H.W. Manner and V.A. Rowe, Department of
Biology, Loyola U. of Chicago, Chicago, 60626.

Pregnant female mice (Jax C57B1/KsJ) were given daily
intramuscular injections of 500 mg/kg amygdalin
throughout the gestation period. Controls were injected
with Locke's solution. Each litter was examined for
size, number of pups, organ malformations, and bone
deformities. In addition, spontaneous abortions, if
they occurred, were noted. Neither the experimental,
nor the control animals showed any abnormalities
above those normally found in mouse litters. Of 88
experimental animals, 1 animal was born dead, 1
exhibited polydactyly and 1 had the l3th rib missing.
All others were normal. In the controls, 1 was born
dead, and 1 had multiple teratogenicites. There was
no significant difference between the control and the
experimental animals indicating that amygdalin in the
daily dosages as high as 50 mg/kg were completely
non-toxic to developing embryos.

***********************************************
ENZYMATIC ANALYSIS OF NORMAL AND MALIGMANT TISSUES,

H.W. Manner, T.L. Michalsen and S.J.
DiSanti, Department of Biology, Loyola U. of Chicago,
Chicago, 60626.

Both B-Glucosidase (E.C. 3.2.1.21) and Rhodanese (E.C. 2.8.1.1.)
are central in the Laetrile theory of cancer therapy.
Concentrations of both enzymes were determined in normal brain, liver
and muscle tissues of non-cancerous C3H/HeJ mice.
These tissues were also analyzed in the experimental
tumor-bearing animals. In addition, the adeno-carcin-
omatous tissue of the mammary gland was studied.
The results, based on 10 animals per group, are charted
below.

        NON-TUMOR BEARING MOUSE
In Units     Brain   Liver    Muscle
------------------------------------
B-Glu.       trace    .33     trace
mg protein

Rhodanese     .53    2.97      .14
mg protein


        TUMOR BEARING MOUSE

In Units     Brain   Liver    Muscle   Tumor
--------------------------------------------
B-Glu.       trace    .54     trace    .84
mg protein

Rhodanese     .17    1.80      .13     .09
mg protein

***********************************************
END PRODUCTS OF AMYGDALIN METABOLISM,

H.W. Manner and M.A. Manhardt, Department of
Biology, Loyola U. of Chicago.

The laetrile hypothesis suggests that Beta-Glucosidase hydrolyzes
amygdalin to laevo-mandelonitrile and then to HCN and benzaldehyde.
Hydrogen cyanide is converted to Rhodanese, in the presence of sodium
thiosulfate to sodium thiocyanate which is excreted in the urine.
Benzaldehyde oxidizes to benzoic acid, which combines with glycine
to become hippuric acid, which is also excreted in the urine. If the
hypothesis is true both NaSCN and hippuric acid should increase in
the urine as the quantity of amygdalin injected inereases. Jax 
C57B1/KsJ mice were injected intramuscularly with 500, 1000, 1500,
2000, 3500 and 5000 mg/kg. As the dose of amygdalin inereased there
was a corresponding increase in both the thiocyanate and hippuric acid
content in the urine, thereby affirming the basic hypothesis.
------------------------------------------------------------------------
                    The ROBERT CATHEY RESEARCH SOURCE.
All pages Copyright © 1996 R.S.Cathey, except where specified otherwise.