Instructor: Michael Riscoe,
PhD
Class Schedule: TR, Noon to 2pm at PSU, SB2, Room 247.
Textbook: Biochemistry by Lubert Stryer, 4th Edition
Questions: Office Number 721-7885 (w/ voice mail)
Office Location: VA Medical Center, Building #101, Room #511;
drop in or call anytime
Examination Schedule & Grading Policy: We will have 4 exams
and your grade will be
determined from the average of the test scores. I will use a curve
if necessary but as
yet have never had to do so.
Tests will cover material presented in class only; you are not responsible
for material in the book which is not presented.
Course Outline
Chapter 1. Prelude. (Or What is biochemist and what has it done for
me lately?)
Proteins
Chapter 2. Protein Structure and Function
amino acids
peptide bonds
primary/secondary/tertiary/quartenary structure
functions
Chapter 3. Exploring proteins
protein purification
sequencing/the Edman degradation
Western blotting
analytical procedures
Nucleic Acids
Chapter 4. DNA and RNA: Molecules of Heredity
-
nucleotides
-
original expts to prove that DNA is the stuff that genes are made of
-
DNA replication
-
the double helix
-
super coiling
Chapter 5. Flow of Genetic Information
Chapter 6. Exploring Genes: Analyzing, Constructing, and Cloning
DNA
-
Restriction enzymes
-
DNA sequencing
-
cloning genes into bacteriaphage and expression.
We will also cover the polymerase chain reaction (PCR).
Back to Proteins
Chapter 7. Portrait of Allosteric Proteins: Myoglobin and Hemoglobin
-
an example of protein conformation
-
dynamics and function
-
molecular mutations and inherited diseases
Chapter 8. Enzymes: Basic Concepts and Kinetics
-
enzyme catalysis, specificity, and regulation.
-
Formation of the enzyme-substrate complex
-
features of active sites
-
hyperbolic substrate/velocity plots
-
Michaelis Menten rate kinetics
-
allosteric enzymes
-
enzyme inhibition
-
therapeutic examples (ethanol and penicillin)
Chapter 9. Catalytic Strategies: Enzyme mechanisms
-
we will probably focus on HIV protease here, depending on the makeup of
the class.
Chapter 10. Regulatory Strategies: control of enzymatic activity
-
overview of the various mechanisms for enzyme control
-
aspartate transcarbamoylase
-
models of allosterism
-
proenzymes
-
thrombin---the clotting cascade
Membranes
Chapter 11. Membrane Structure and Dynamics --we'll focus on:
-
the Fluid Mosaic model
-
components of membranes
-
lipid bilayers
-
semi-permeable nature
-
glycoproteins
Chapter 13. Signal Transduction Cascade -we'll focus on:
-
bacterial chemotaxis: how do bacteria know which direction to swim?
Chapter 17. Metabolism-Basic concepts and design
-
the central nature of ATP
-
the role of vitamins, and
-
general scheme for extraction of energy from food stuffs
Chapter 18. Carbohydrates-Glycogen etc
Chapter 19. Glycolysis
Integration of Research Activities-
"What sorts of things do Biochemists getpaid to do?"
Chapter 20. The Citric Acid cycle.
Chapter 21. Oxidative Phosphorylation and the ultimate diet pill
Chapter 22. The Pentose Phosphate Pathway and Gluconeogenesis
Current page: http://www.navi.net/~rsc/pchem/syl.htm
Introduction
Prot01.htm
Prot02.htm -- Key concepts, review
Prot03.htm
Prot04.htm
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