C:\europa11\eudora2\trophref.txt

01]
=================================================================
Gal, David, and Friedman, Michael, Follow-Up and Contraception, in
Gestational Trophoblast Disease, Chapter 18, page 179-186, Ed.s Szulman,
A.E., and Buchsbaum, H.J., Springer-Verlag, 1987.

"Thyroid Function
"The clinical association of hyperthyroidism with trophoblastic tumors has
 been documented [4]". p. 180.

get:][4]  Surwit EA, Hammond CB. Gestational trophoblastic neoplasia. The
     year book of Obstetrics and Gynecology. Chicago, London, Year Book
     Medical Publishers, 1980; p. 275.

"Birth Control
"A pregnancy in the follow-up period should be avoided, since raising hCG
levels fail to differentiate a normal pregnancy from persistent
trophoblastic disease." p. 182.
===================================================================
02]
---
a)hCG is ~34% carbohydrate by weight of the protein (Lapthorn AJ, Harris DC,
  et al., Crystal Structure of hCG, Nature, 369(9):455-461,9 June, 1994)
  p.458:Carbohydrate structure.

b)op.cit:p. 460: Carboxypeptidase digestion of both hCGalpha and LHalpha,
                 although not detrimental to subunit assembly, essentially
                 eliminates receptor binding(27,28)

c)rsc: Use of Carboxypeptidase might be used to introduce enzyme therapy
       with less severe reactions.

d)pg 461: deglycosylated hCG can be converted back into an active form when
          bound to polyclonal antibodies(31,37).

e)rsc: evidence of proteolytic and amylolytic action: "Whereas hCG is found
       mainly as a heterodimer in the early stages of pregnancy, in the
       later stages both subunits are found in large quantities as separate
       molecules.  Free alpha-subunit is more heavily glycosylated and has
       hormonal activity(38). Smaller forms of the Beta-subunit that have
       been considered degradation products are found. The smallest of these,
       Beta-core(39), is composed of two polypeptides, Beta6-40 and
       Beta55-92, which arise from excision of 15 residues from the long
       loop as well as truncation at the N and C termini. The structural
       similarity of this truncated Beta-molecule with PDGF is striking and
       suggests that the circulating Beta-core molecule has a defined
       tertiary structure and perhaps a biological function."
End rsc note (e).
-----
3]
Found in Gonadotropins, Eds. Saxena, Beling, and Gandy, Wilely Interscience,
1972.

Ross GT, van Hall EV, Vaitukaitis JL, Braunstein GD and Rayford PL,
Chapter 32.: Sialic Acid and Immunologic and Biologic Activity of 
Gonadotropins, pp 417-423
And following chapters, and discussions.(titles not noted, page numbers
noted).

Present Address (Hall), Department of Obstetrics and Gynecology, University
of Nijmegen, Nijmegen, The Netherlands.

04]
rsc notes: a)Clostridium perfringens neuraminidase (Worthington, Sigma).
           Define, characterize clostridium p.  Ingestible? Availability?
           b)Test effects of amygdalin and amygdalin/beta-glucosidase on
           amylase starch digestion.

05]Trypsin inhibition after partial and complete desialylation; partial and
complete carbohydrate removal by amylase.

a)Amylase-sialic acid interaction.
b)Trypsin-sialic acid interaction.

06]Desialylation results in exposure of galactosyl residues --resulting in
   "avid hepatic uptake, and rapid disappearance of some ofthese substances
   from plasma of rats -page 421, Ross, et al.

07]Antigenic properties not possessed by desialylated hCG.-p 420. Table.III.
-----
get:]Currie & Bagshawe, The masking of antigens on trophoblast and cancer
     cells, Lancet, i:708, 1967.

get:]Vassar, PS, The electric charge density of human tumor cell surfaces,
     Lab. Invest. 12:1072, 1963.
--------------
08]
hCG Carbohydrate composition (Om.P.Bahl, in: Gonadotropins: Studies on the
Primary Structure of hCG and its Relationship to other Glycoprotein
Hormones)

(residues 28,000 molecular) [according to table II, p. 202]

L-fucose      (1)
D-mannose     (9)
D-galactose   (9)
Glucosamine  (11)
Galactosamine (3)
sialic acid   (8)

2-acetamido-2-deoxy-D-glucose & 2-acetomido-2-deoxy-D-galactose (?)

On p. 200, he listed fucose, galactose, mannose, and the above acetamindo-
forms, as well as the rotations in the table quoted.  Not sure if these form
galactosamine or glucosamine.